Comparative Study Seminiferous tubule fluid secretion is a Sertoli cell microtubule-dependent process inhibited by 2,5-hexanedione exposure J H Richburg et al. Toxicol Appl Pharmacol. 1994 Oct.
Abstract Mammalian Sertoli cells are responsible for the formation and secretion of seminiferous tubule fluid (STF) which provides the nutritional and hormonal microenvironment necessary for spermatogenesis. Exposure of rats to 2,5-hexanedione (2,5-HD) results in testicular injury characterized by a decrease of STF secretion which immediately precedes bulk germ cell necrosis. The earliest biochemical change in 2,5-HD-exposed rats is an alteration in
testicular microtubule assembly kinetics. In this study, we investigate the relationship between microtubule-dependent processes and STF secretion in adult Sprague-Dawley CD rats by exposing seminiferous tubules to two types of toxicants: (1) those which alter microtubules (colchicine and 2,5-HD) and (2) an inhibitor of protein secretion (brefeldin A, [BFA]). Secretion of STF is quantitated by monitoring the rate of transport of a microinjected oil droplet in the lumen of isolated seminiferous
tubules using time-lapse stereoscopic microscopy. The rate of oil droplet transport in seminiferous tubules isolated from testis pretreated in vivo for 2 hr with colchicine (40 micrograms/testis) was significantly decreased. Exposure of isolated seminiferous tubules to BFA (10 micrograms/ml) for 40 min also significantly decreased the transport of lumenal oil droplets. Exposure of rats to 1%, 2,5-HD in drinking water decreased transport of injected oil droplets in seminiferous tubules beginning
at 3 weeks of exposure in the absence of significant alterations in testicular morphology. These data demonstrate that normal STF secretion requires an intact, microtubule-dependent intracellular membrane transport pathway and strengthen the association between 2,5-HD-induced disruption of Sertoli cell STF secretion and 2,5-HD-induced alterations in Sertoli cell microtubules. Similar articles 2,5-Hexanedione exposure alters the rat Sertoli cell cytoskeleton. I. Microtubules and seminiferous tubule fluid secretion. Johnson KJ, Hall ES, Boekelheide K. Johnson KJ, et al. Toxicol Appl Pharmacol. 1991 Dec;111(3):432-42. doi:
10.1016/0041-008x(91)90248-d. Toxicol Appl Pharmacol. 1991. PMID: 1746024 2,5-hexanedione and carbendazim coexposure synergistically disrupts rat spermatogenesis despite opposing molecular effects on
microtubules. Markelewicz RJ Jr, Hall SJ, Boekelheide K. Markelewicz RJ Jr, et al. Toxicol Sci. 2004 Jul;80(1):92-100. doi: 10.1093/toxsci/kfh240. Epub 2004 May 12. Toxicol Sci. 2004. PMID: 15141104 2,5-Hexanedione exposure alters microtubule motor distribution in adult rat testis. Hall ES, Hall SJ, Boekelheide K. Hall ES, et al. Fundam Appl Toxicol. 1995 Feb;24(2):173-82. doi: 10.1006/faat.1995.1021. Fundam Appl Toxicol.
1995. PMID: 7737429 Spermatogenesis by Sisyphus: proliferating stem germ cells fail to repopulate the testis after 'irreversible' injury. Boekelheide K, Schoenfeld HA. Boekelheide K, et
al. Adv Exp Med Biol. 2001;500:421-8. doi: 10.1007/978-1-4615-0667-6_64. Adv Exp Med Biol. 2001. PMID: 11764975 Review. Cell-cell interactions in the control of spermatogenesis as studied
using Leydig cell destruction and testosterone replacement. Sharpe RM, Maddocks S, Kerr JB. Sharpe RM, et al. Am J Anat. 1990 May;188(1):3-20. doi: 10.1002/aja.1001880103. Am J Anat. 1990. PMID: 2161173 Review.
Cited by Docetaxel-induced maculopathy possibly potentiated by concurrent hydroxychloroquine use. Elhusseiny AM, Relhan N, Smiddy WE. Elhusseiny AM, et al. Am J Ophthalmol Case Rep. 2019 Sep 26;16:100560. doi: 10.1016/j.ajoc.2019.100560. eCollection 2019
Dec. Am J Ophthalmol Case Rep. 2019. PMID: 31650088 Free PMC article. Expression of testicular phosphorylated proteins in types 1 and 2 diabetes mellitus in mice: An experimental study. Sampannang
A, Arun S, Burawat J, Sukhorum W, Iamsaard S. Sampannang A, et al. Int J Reprod Biomed. 2019 Sep 3;17(8):567-576. doi: 10.18502/ijrm.v17i8.4822. eCollection 2019 Aug. Int J Reprod Biomed. 2019. PMID: 31583374 Free PMC article. Leukemia Inhibitory Factor-Receptor is Dispensable for Prenatal Testis Development but is Required in Sertoli cells for Normal Spermatogenesis in Mice. Curley M, Milne L, Smith S, Atanassova N, Rebourcet D, Darbey A, Hadoke PWF, Wells S, Smith LB. Curley M, et
al. Sci Rep. 2018 Aug 1;8(1):11532. doi: 10.1038/s41598-018-30011-w. Sci Rep. 2018. PMID: 30068994 Free PMC article. Glucose and glutamine handling in the Sertoli cells of transgenic rats
overexpressing regucalcin: plasticity towards lactate production. Mateus I, Feijó M, Espínola LM, Vaz CV, Correia S, Socorro S. Mateus I, et al. Sci Rep. 2018 Jul 9;8(1):10321. doi: 10.1038/s41598-018-28668-4. Sci Rep. 2018. PMID: 29985416 Free PMC article. Reproductive toxicity of 2,5-hexanedione in male rats. Aoki K, Kihaile PE, Misumi J, Pei W, Kudo M. Aoki K, et al. Reprod Med Biol. 2004 May 20;3(2):59-62. doi: 10.1111/j.1447-0578.2004.00053.x. eCollection 2004 Jun. Reprod Med Biol.
2004. PMID: 29699185 Free PMC article.
Publication types MeSH terms Substances Grant support - K04 ES00193/ES/NIEHS NIH HHS/United States
- R01 ES05033/ES/NIEHS NIH HHS/United States
- T32 ES 07272/ES/NIEHS NIH HHS/United States
|
